Modification of Tobacco rattle virus RNA1 to Serve as a VIGS Vector Reveals That the 29K Movement Protein Is an RNA Silencing Suppressor of the Virus
Tobacco rattle virus (TRV) has a bipartite, positive-sense single-stranded RNA genome and is widely used for virus-induced gene silencing (VIGS) in plants.RNA1 of TRV that lacks the gene for the cysteine-rich 16K silencing-suppression protein here infects plants systemically in the absence of RNA2.Here, we attempted to engineer RNA1 for use as a VI